Cell uptake survey of pegylated nanographene oxide.
Nanotechnology
; 23(46): 465103, 2012 Nov 23.
Article
em En
| MEDLINE
| ID: mdl-23093209
ABSTRACT
Graphene and more specifically, nanographene oxide (GO) has been proposed as a highly efficient antitumoral therapy agent. Nevertheless, its cell uptake kinetics, its influence in different types of cells and the possibility of controlling cellular internalization timing, is still a field that remains unexplored. Herein, different cell types have been cultured in vitro for several incubation periods in the presence of 0.075 mg ml(-1) pegylated GO solutions. GO uptake kinetics revealed differences in the agent's uptake amount and speed as a function of the type of cell involved. Osteoblast-like cells GO uptake is higher and faster without resulting in greater cell membrane damage. Moreover, the dependence on the commonly used PEG nature (number of branches) also influences the viability and cell uptake speed. These facts play an important role in the future definition of timing parameters and selective cell uptake control in order to achieve an effective therapy.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Polietilenoglicóis
/
Nanoestruturas
/
Grafite
Limite:
Animals
/
Humans
Idioma:
En
Revista:
Nanotechnology
Ano de publicação:
2012
Tipo de documento:
Article
País de afiliação:
Espanha