Discovery of novel integrin ligands from combinatorial libraries using a multiplex "beads on a bead" approach.
Nano Lett
; 12(11): 5957-65, 2012 Nov 14.
Article
em En
| MEDLINE
| ID: mdl-23094984
ABSTRACT
The development of screening approaches to identify novel affinity ligands has paved the way for a new generation of molecular targeted nanomedicines. Conventional methods typically bias the display of the target protein to ligands during the screening process. We have developed an unbiased multiplex "beads on a bead" strategy to isolate, characterize, and validate high affinity ligands from OBOC libraries. Novel non-RGD peptides that target α(v)ß(3) integrin were discovered that do not affect cancer or endothelial cell biology. The peptides identified here represent novel integrin-targeted agents that can be used to develop targeted nanomedicines without the risk of increased tumor invasion and metastasis.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Técnicas de Química Combinatória
Limite:
Humans
Idioma:
En
Revista:
Nano Lett
Ano de publicação:
2012
Tipo de documento:
Article