Secretion of ATP from Schwann cells through lysosomal exocytosis during Wallerian degeneration.
Biochem Biophys Res Commun
; 429(3-4): 163-7, 2012 Dec 14.
Article
em En
| MEDLINE
| ID: mdl-23142593
ABSTRACT
The present study demonstrates that adenosine triphosphate (ATP) is released from Schwann cells through lysosomal exocytosis during Wallerian degeneration and in response to stimulation. In primary Schwann cell cultures, ATP was stored in lysosomal vesicles. ATP could then induce Ca(2+)-dependent lysosomal exocytosis. Among three stimulants of lysosomal exocytosis (glutamate, NH(4)Cl and zymosan), only NH(4)Cl was sufficient to induce ATP release from ex vivo sciatic nerve explants at 3 days in vitro. Lysosomal exocytosis inhibitors (metformin, chlorpromazine and vacuolin-1) reversed the effect of NH(4)Cl-enhanced ATP release, replicating the state of explants treated with NH(4)Cl in the absence of lysosomal exocytosis inhibitors. Furthermore, we observed ATP release through lysosomal exocytosis during Wallerian degeneration in sciatic explant cultures using the recently identified vesicular nucleotide transporter (VNUT). From these experiments, we conclude that the exocytosis of lysosomes in Schwann cells during Wallerian degeneration is Ca(2+)-dependent, and that it induces ATP release from Schwann cells.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Células de Schwann
/
Degeneração Walleriana
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Trifosfato de Adenosina
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Exocitose
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Lisossomos
Limite:
Animals
Idioma:
En
Revista:
Biochem Biophys Res Commun
Ano de publicação:
2012
Tipo de documento:
Article