Systematic reconstruction of RNA functional motifs with high-throughput microfluidics.
Nat Methods
; 9(12): 1192-4, 2012 Dec.
Article
em En
| MEDLINE
| ID: mdl-23142872
ABSTRACT
We present RNA-mechanically induced trapping of molecular interactions (RNA-MITOMI), a microfluidic platform that allows integrated synthesis and functional assays for programmable RNA libraries. The interaction of a comprehensive library of RNA mutants with stem-loop-binding protein precisely defined the RNA structural and sequence features that govern affinity. The functional motif reconstructed in a single experiment on our platform uncovers new binding specificities and enriches interpretation of phylogenetic data.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
RNA
/
Microfluídica
/
Motivos de Nucleotídeos
Limite:
Animals
/
Humans
Idioma:
En
Revista:
Nat Methods
Assunto da revista:
TECNICAS E PROCEDIMENTOS DE LABORATORIO
Ano de publicação:
2012
Tipo de documento:
Article
País de afiliação:
Estados Unidos