Comparison of five methods for oxacillin susceptibility testing of Staphylococcus aureus isolates from cystic fibrosis patients.
Rev Inst Med Trop Sao Paulo
; 54(6): 305-6, 2012 Dec.
Article
em En
| MEDLINE
| ID: mdl-23152311
ABSTRACT
Methicillin-resistant Staphylococcus aureus (MRSA) are now a worldwide problem. Cystic fibrosis (CF) patients are commonly colonized and infected by MRSA. Accurate oxacillin susceptibility testing is mandatory for the adequate management of these patients. We performed a comparison of the accuracy of different tests in CF isolates, including methicillin-susceptible S. aureus and MRSA with different SCCmec types, and using the mecA gene as the gold-standard. The sensitivity and specificity of oxacillin disc, Etest, and oxacillin agar screening plate were 100%. Sensitivity of the cefoxitin disc was 85% and specificity was 100%. For clinically relevant isolates, laboratories may consider the use of a combination of two phenotypic methods.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Oxacilina
/
Infecções Estafilocócicas
/
Staphylococcus aureus
/
Proteínas de Bactérias
/
Fibrose Cística
/
Antibacterianos
Tipo de estudo:
Diagnostic_studies
Limite:
Humans
Idioma:
En
Revista:
Rev Inst Med Trop Sao Paulo
Ano de publicação:
2012
Tipo de documento:
Article
País de afiliação:
Brasil