Image analysis tools to quantify cell shape and protein dynamics near the leading edge.
Cell Struct Funct
; 38(1): 1-7, 2013.
Article
em En
| MEDLINE
| ID: mdl-23165752
ABSTRACT
We present a set of flexible image analysis tools to analyze dynamics of cell shape and protein concentrations near the leading edge of cells adhered to glass coverslips. Plugins for ImageJ streamline common analyses of microscopic images of cells, including the calculation of leading edge speeds, total and average intensities of fluorescent markers, and retrograde flow rate measurements of fluorescent single-molecule speckles. We also provide automated calculations of auto- and cross-correlation functions between velocity and intensity measurements. The application of the methods is illustrated on images of XTC cells.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Processamento de Imagem Assistida por Computador
/
Proteínas
/
Forma Celular
Limite:
Humans
Idioma:
En
Revista:
Cell Struct Funct
Ano de publicação:
2013
Tipo de documento:
Article
País de afiliação:
Estados Unidos