The apoptotic transcriptome of the human MII oocyte: characterization and age-related changes.
Apoptosis
; 18(2): 201-11, 2013 Feb.
Article
em En
| MEDLINE
| ID: mdl-23179180
ABSTRACT
Fully competent oocytes represent the final outcome of a highly selective process. The decline of oocyte competence with ageing, coupled to quantitative decrease of ovarian follicles has been well established; on the contrary, its molecular bases are still poorly understood. Through quantitative high throughput PCR, we investigated the role of apoptotic machinery (AM) in this process. To this aim, we determined AM transcriptome in mature MII oocyte pools from women aged more than 38 years (cohort A), and compared to women aged up to 35 years (cohort B). Subsequently, 10 representative AM genes were selected and analyzed in 33 single oocytes (15 from cohort A and 18 from cohort B). These investigations led us to identify (1) the significant upregulation of proapoptotic genes such us CD40, TNFRSF10A, TNFRSF21 and the downregulation of antiapoptotic genes such as BCL2 and CFLAR in cohort A respect to cohort B; (2) AM transcripts that have not previously been reported in human oocytes (BAG3, CD40, CFLAR, TNFRSF21, TRAF2, TRAF3). Our results demonstrated that during maturation the oocytes from older women selectively accumulate mRNAs that are able to trigger the extrinsic apoptotic pathway. These data contribute to clarify the molecular mechanisms of AM involvement in the natural selection strategy of removing low quality oocytes and preventing unfit or poorly fit embryos.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Oócitos
/
Envelhecimento
/
Apoptose
/
Receptores do Fator de Necrose Tumoral
/
Antígenos CD40
/
Proteínas Proto-Oncogênicas c-bcl-2
/
Proteína Reguladora de Apoptosis Semelhante a CASP8 e FADD
/
Receptores do Ligante Indutor de Apoptose Relacionado a TNF
/
Transcriptoma
Tipo de estudo:
Prognostic_studies
Limite:
Adult
/
Female
/
Humans
Idioma:
En
Revista:
Apoptosis
Ano de publicação:
2013
Tipo de documento:
Article
País de afiliação:
Itália