Flow-through comb electroporation device for delivery of macromolecules.
Anal Chem
; 85(3): 1637-41, 2013 Feb 05.
Article
em En
| MEDLINE
| ID: mdl-23259401
ABSTRACT
We present a microfluidic electroporation device with a comb electrode layout fabricated in polydimethylsiloxane (PMDS) and glass. Characterization experiments with HeLa cells and fluorescent dextran show efficient delivery (â¼95%) with low toxicity (cell viability â¼85%) as well as rapid pore closure after electroporation. The activity of delivered molecules is also verified by silencing RNA (siRNA) studies that demonstrate gene knockdown in GFP expressing cells. This simple, scalable approach to microfluidic, flow-through electroporation could facilitate the integration of electroporation modules within cell analysis devices that perform multiple operations.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Técnicas de Transferência de Genes
/
Eletroporação
/
Substâncias Macromoleculares
Limite:
Humans
Idioma:
En
Revista:
Anal Chem
Ano de publicação:
2013
Tipo de documento:
Article
País de afiliação:
Estados Unidos