Monitoring ion activities in and around cells using ion-selective liquid-membrane microelectrodes.
Sensors (Basel)
; 13(1): 984-1003, 2013 Jan 15.
Article
em En
| MEDLINE
| ID: mdl-23322102
ABSTRACT
Determining the effective concentration (i.e., activity) of ions in and around living cells is important to our understanding of the contribution of those ions to cellular function. Moreover, monitoring changes in ion activities in and around cells is informative about the actions of the transporters and/or channels operating in the cell membrane. The activity of an ion can be measured using a glass microelectrode that includes in its tip a liquid-membrane doped with an ion-selective ionophore. Because these electrodes can be fabricated with tip diameters that are less than 1 µm, they can be used to impale single cells in order to monitor the activities of intracellular ions. This review summarizes the history, theory, and practice of ion-selective microelectrode use and brings together a number of classic and recent examples of their usefulness in the realm of physiological study.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Membrana Celular
/
Eletrodos Seletivos de Íons
/
Íons
Limite:
Animals
Idioma:
En
Revista:
Sensors (Basel)
Ano de publicação:
2013
Tipo de documento:
Article
País de afiliação:
Estados Unidos