Comparing TCR beta chain variable gene profile skewness between children with tuberculosis and BCG-vaccinated children.
Arch Iran Med
; 16(2): 104-8, 2013 Feb.
Article
em En
| MEDLINE
| ID: mdl-23360633
ABSTRACT
BACKGROUND:
We compared the T cell antigen receptor (TCR-BV) gene families of peripheral blood mononuclear cells (PMBC) between children with tuberculosis (TB) and those inoculated with the Bacille Calmette Guerin (BCG) vaccine.METHODS:
The total RNA was extracted from PMBC of 15 TB children, 15 BCG-vaccinated children and 15 healthy controls. The RNAs were reverse-transcribed and amplified by polymerase chain reaction (PCR). PCR products were separated on 1.5% agarose gel and analyzed with the Genescan technique.RESULTS:
Some TCR-BV gene families in TB children and BCG-vaccinated children exhibited a blur band in the predicted position on 1.5% agarose gel, some showed a distinct or fainted band. In general, many shared predominant clonal TCR-BV gene families (Vß2, Vß16, Vß21, Vß22) and the restricted-expression families (Vß14 and Vß17). All the gene families of the control children only exhibited blur bands and polyclonal.CONCLUSIONS:
The skewed profile of TCR-BV gene families in TB children and BCG-vaccinated children are similar, which may probably explain the protective effects of BCG-vaccine against TB in children.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Tuberculose Pulmonar
/
Vacina BCG
/
Genes Codificadores da Cadeia beta de Receptores de Linfócitos T
Tipo de estudo:
Observational_studies
/
Prognostic_studies
/
Risk_factors_studies
Limite:
Child, preschool
/
Humans
Idioma:
En
Revista:
Arch Iran Med
Ano de publicação:
2013
Tipo de documento:
Article
País de afiliação:
China