Signal recognition initiates reorganization of the presequence translocase during protein import.
EMBO J
; 32(6): 886-98, 2013 Mar 20.
Article
em En
| MEDLINE
| ID: mdl-23403928
ABSTRACT
The mitochondrial presequence translocase interacts with presequence-containing precursors at the intermembrane space (IMS) side of the inner membrane to mediate their translocation into the matrix. Little is known as too how these matrix-targeting signals activate the translocase in order to initiate precursor transport. Therefore, we analysed how signal recognition by the presequence translocase initiates reorganization among Tim-proteins during import. Our analyses revealed that the presequence receptor Tim50 interacts with Tim21 in a signal-sensitive manner in a process that involves the IMS-domain of the Tim23 channel. The signal-driven release of Tim21 from Tim50 promotes recruitment of Pam17 and thus triggers formation of the motor-associated form of the TIM23 complex required for matrix transport.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas de Membrana Transportadoras
/
Sinais Direcionadores de Proteínas
/
Proteínas de Transporte
/
Transporte Proteico
Idioma:
En
Revista:
EMBO J
Ano de publicação:
2013
Tipo de documento:
Article
País de afiliação:
Alemanha