Pituitary tumor transforming gene and insulin-like growth factor 1 receptor expression and immunohistochemical measurement of Ki-67 as potential prognostic markers of pituitary tumors aggressiveness.
Endocrinol Nutr
; 60(7): 358-67, 2013.
Article
em En
| MEDLINE
| ID: mdl-23416216
ABSTRACT
INTRODUCTION AND OBJECTIVE:
The ability to predict recurrence of pituitary adenoma (PA) after surgery may be helpful to determine follow-up frequency and the need for adjuvant treatment. The purpose of this study was to assess the prognostic capacity of pituitary tumor transforming gene (PTTG), insulin-like growth factor 1 receptor (IGF1R), and Ki-67. MATERIALS ANDMETHODS:
In this retrospective study, the normalized copy number (NCN) of PTIG and IGF1R mRNA was measured using RT-PCR, and the Ki-67 index was measured by immunohistochemistry in 46 PA samples. Clinical data, histological subtype, and radiographic characteristics were collected to assess associations between variables and tumor behavior. Progression of tumor remnants and its association to markers was also studied in 14 patients with no adjuvant treatment after surgery followed up for 46±36 months.RESULTS:
Extrasellar tumors had a lower PTTG expression as compared to sellar tumors (0.065 [1st-3rd quartile 0.000-0.089] NCN vs. 0.135 [0.105-0.159] NCN, p=0.04). IGF1R expression changed depending on histological subtype (p=0.014), and was greater in tumor with remnant growth greater than 20% during follow-up (10.69±3.84 NCN vs. 5.44±3.55 NCN, p=0.014).CONCLUSIONS:
Our results suggest that the IGF1R is a more helpful molecular marker than PTTG in PA management. Ki-67 showed no association to tumor behavior. However, the potential of these markers should be established in future studies with standardized methods and on larger samples.Palavras-chave
ACTH; Adenoma hipofisario; Antígeno Ki-67; CT; DA; FFPE; FPA; GH; GT; Humanos; Humans; IGF1R; Insulin-like growth factor 1 receptor; Ki-67 antigen; LT; MAPK; MRI; NC; NFPA; PA; PI3K/Akt; PRL; PTTG; Pituitary adenoma; Pituitary tumor transforming gene protein; Proteína del gen transformador de tumores hipofisarios; ROC; RT-PCR; Receptor del factor de crecimiento insulinoide 1; SSa; ST; TSH; TT; Thyroid stimulating hormone; Thyrotrophic adenomas; adrenocorticotropic hormone; corticotrophic adenomas; dopamine agonist; formalin-fixes and paraffin-embedded; functioning pituitary adenoma; gonadotrophic adenomas; growth hormone; insulin-like growth factor 1 receptor; lactotrophic adenomas; magnetic resonance imaging; mitogen-activated protein kinases; non-functioning pituitary adenoma; null-cell adenomas; phosphoinositide 3-kinase/Akt pathway; pituitary adenomas; pituitary tumor transforming gene; prolactin; real time polymerase chain reaction; receiver operating characteristics; somatostatin analogs; somatotrophic adenomas
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Neoplasias Hipofisárias
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Biomarcadores Tumorais
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Receptor IGF Tipo 1
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Antígeno Ki-67
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Securina
/
Proteínas de Neoplasias
Tipo de estudo:
Observational_studies
/
Prognostic_studies
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Risk_factors_studies
Idioma:
En
Revista:
Endocrinol Nutr
Assunto da revista:
CIENCIAS DA NUTRICAO
/
ENDOCRINOLOGIA
Ano de publicação:
2013
Tipo de documento:
Article
País de afiliação:
Espanha