Structural characterization of an immunoenhancing heteroglycan of a hybrid mushroom (pfls1h) of Pleurotus florida and Lentinus squarrosulus (Mont.) Singer.
Carbohydr Res
; 371: 45-51, 2013 Apr 19.
Article
em En
| MEDLINE
| ID: mdl-23500959
ABSTRACT
An immunostimulating water-soluble heteroglycan (PS-II) was isolated from an aqueous extract of the fruit bodies of a hybrid mushroom, pfls1h produced by intergeneric protoplast fusion between Pleurotus florida and Lentinus squarrosulus (Mont.) Singer. Structural characterization of PS-II was carried out using sugar analysis, methylation experiment, periodate oxidation and 1D/2D NMR studies. Sugar analysis indicated the presence of glucose, mannose and galactose in a molar ratio of 112. Methylation analysis revealed that PS-II was composed of (1â6)- and (1â2,4,6)-α-D-galactopyranosyl, terminal ß-D-mannopyranosyl and terminal ß-D-glucopyranosyl residues in a relative proportion of approximately 1111. On the basis of chemical analysis and NMR studies, the structure of the repeating unit of the heteroglycan was established to consist of a backbone chain of two (1â6)-α-D-galactopyranosyl residues, one of which is branched at O-2 with terminal ß-D-Manp and at O-4 with terminal ß-D-Glcp. This heteroglycan (PS-II) showed in vitro splenocyte, thymocyte and macrophage activations.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Polissacarídeos
/
Lentinula
/
Pleurotus
/
Carpóforos
/
Fatores Imunológicos
Limite:
Animals
Idioma:
En
Revista:
Carbohydr Res
Ano de publicação:
2013
Tipo de documento:
Article
País de afiliação:
Índia