Comparison of four multiplex PCR assays for the detection of viral pathogens in respiratory specimens.
J Virol Methods
; 191(2): 118-21, 2013 Aug.
Article
em En
| MEDLINE
| ID: mdl-23583489
ABSTRACT
Multiplex PCR has become the test of choice for the detection of multiple respiratory viruses in clinical specimens. However, there are few direct comparisons of different PCR assays. This study compares 4 different multiplex PCR assays for the recovery of common respiratory viruses. We tested 213 respiratory specimens using four different multiplex PCR assays the xTAG respiratory viral panel fast (Abbott Molecular Laboratories), Fast-track Respiratory Pathogen assay (Fast-track Diagnostics), Easyplex respiratory pathogen 12 kit (Ausdiagnostics), and an in-house multiplex real-time PCR assay. The performance of the four assays was very similar, with 93-100% agreement for all comparisons. Other issues, such as through-put, technical requirements and cost, are likely to be as important for making a decision about which of these assays to use given their comparative performance.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Infecções Respiratórias
/
Virologia
/
Vírus
/
Viroses
/
Técnicas de Diagnóstico Molecular
/
Reação em Cadeia da Polimerase Multiplex
Tipo de estudo:
Diagnostic_studies
/
Evaluation_studies
/
Prognostic_studies
Limite:
Humans
Idioma:
En
Revista:
J Virol Methods
Ano de publicação:
2013
Tipo de documento:
Article
País de afiliação:
Nova Zelândia