Fibronectin is essential for reparative cardiac progenitor cell response after myocardial infarction.
Circ Res
; 113(2): 115-25, 2013 Jul 05.
Article
em En
| MEDLINE
| ID: mdl-23652800
ABSTRACT
RATIONALE Adoptive transfer of cardiac progenitor cells (CPCs) has entered clinical application, despite limited mechanistic understanding of the endogenous response after myocardial infarction (MI). Extracellular matrix undergoes dramatic changes after MI and therefore might be linked to CPC-mediated repair. OBJECTIVE:
To demonstrate the significance of fibronectin (Fn), a component of the extracellular matrix, for induction of the endogenous CPC response to MI. METHODS ANDRESULTS:
This report shows that presence of CPCs correlates with the expression of Fn during cardiac development and after MI. In vivo, genetic conditional ablation of Fn blunts CPC response measured 7 days after MI through reduced proliferation and diminished survival. Attenuated vasculogenesis and cardiogenesis during recovery were evident at the end of a 12-week follow-up period. Impaired CPC-dependent reparative remodeling ultimately leads to continuous decline of cardiac function in Fn knockout animals. In vitro, Fn protects and induces proliferation of CPCs via ß1-integrin-focal adhesion kinase-signal transducer and activator of transcription 3-Pim1 independent of Akt.CONCLUSIONS:
Fn is essential for endogenous CPC expansion and repair required for stabilization of cardiac function after MI.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Células-Tronco
/
Diferenciação Celular
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Fibronectinas
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Miócitos Cardíacos
/
Infarto do Miocárdio
Limite:
Animals
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Female
/
Humans
Idioma:
En
Revista:
Circ Res
Ano de publicação:
2013
Tipo de documento:
Article
País de afiliação:
Estados Unidos