Does platelet-rich plasma enhance microfracture treatment for chronic focal chondral defects? An in-vivo study performed in a rat model.
Acta Orthop Traumatol Turc
; 47(3): 201-7, 2013.
Article
em En
| MEDLINE
| ID: mdl-23748620
ABSTRACT
OBJECTIVE:
The purpose of the present study was to compare the effectiveness of platelet-rich plasma (PRP) + microfracture and microfracture treatments in the healing of chronic focal chondral defects.METHODS:
The study included 57 adult male Sprague-Dawley rats. Forty-two rats were divided into three groups of 14 rats with a chondral defect (control, microfracture only, PRP+microfracture). The remaining 15 rats were used to produce the PRP preparation. The rats were then euthanatized at 3 and 6 weeks after treatment and examined. Histological analysis using the modified Pineda scoring system and immunohistochemical staining for Type 2 collagen were performed.RESULTS:
At both time intervals, control group histological scores (Week 3 8.8±1.2, Week 6 8.5±0.7) were higher than microfracture (Week 3 6.8±1.0, Week 6 7.1±0.6) and PRP+microfracture (Week 3 6.4±1.3, Week 6 5.7±1.2) scores (p<0.05). The microfracture group score was higher at Week 6 than the PRP+microfracture group (p<0.05). The degree of Type 2 collagen staining was higher at Week 6 in the PRP+microfracture group and was unique in showing staining at the cell membrane.CONCLUSION:
The addition of PRP application to microfracture treatment appears to enhance cartilage healing in chronic focal chondral defects.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Regeneração Óssea
/
Cartilagem Articular
/
Plasma Rico em Plaquetas
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
En
Revista:
Acta Orthop Traumatol Turc
Assunto da revista:
ORTOPEDIA
/
TRAUMATOLOGIA
Ano de publicação:
2013
Tipo de documento:
Article
País de afiliação:
Turquia