Effects of micro environmental factors on natural killer activity (NK) of beta thalassemia major patients.
Cell Immunol
; 282(2): 93-9, 2013 Apr.
Article
em En
| MEDLINE
| ID: mdl-23770717
ABSTRACT
The physiological mechanisms of decreased NK activity of ß-Thalassemia major (BTM) patients are unknown. To assess in vitro effects of mononuclear cells and their cytokine secretion on NK activity, we compared activator receptor levels and cytotoxic activity of purified NK cells and NK cells in mononuclear cells (MNC) pools. We collected cell supernatant from unincubated and incubated MNC with K562 cells and measured their secreted cytokines levels. CD16 was lower on the surface of NK cells in MNC pools from BTM patients compared to healthy volunteers. This inhibition does not appear when NK cells were purified. NKp30 levels in NK cells decreased both as purified cells and as part of a pool of MNC in BTM patients. After incubation of MNC pools with K562 target cells, we found that supernatant levels of IL10, TGFß1 and IL15 cytokines were also significantly higher in BTM patients compared to healthy volunteers.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Leucócitos Mononucleares
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Células Matadoras Naturais
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Talassemia beta
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Microambiente Celular
Idioma:
En
Revista:
Cell Immunol
Ano de publicação:
2013
Tipo de documento:
Article
País de afiliação:
Turquia