A microfluidic approach for protein structure determination at room temperature via on-chip anomalous diffraction.
Lab Chip
; 13(16): 3183-7, 2013 Aug 21.
Article
em En
| MEDLINE
| ID: mdl-23828485
ABSTRACT
We report a microfluidic approach for de novo protein structure determination via crystallization screening and optimization, as well as on-chip X-ray diffraction data collection. The structure of phosphonoacetate hydrolase (PhnA) has been solved to 2.11 Åvia on-chip collection of anomalous data that has an order of magnitude lower mosaicity than what is typical for traditional structure determination methods.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Temperatura
/
Cristalografia por Raios X
/
Técnicas Analíticas Microfluídicas
/
Fosfatase Alcalina
Idioma:
En
Revista:
Lab Chip
Assunto da revista:
BIOTECNOLOGIA
/
QUIMICA
Ano de publicação:
2013
Tipo de documento:
Article
País de afiliação:
Estados Unidos