Concurrent obtaining of aromatic (R)-2-hydroxyacids and aromatic 2-ketoacids by asymmetric oxidation with a newly isolated Pseudomonas aeruginosa ZJB1125.
J Biotechnol
; 167(3): 271-8, 2013 Sep 10.
Article
em En
| MEDLINE
| ID: mdl-23831556
ABSTRACT
Pseudomonas aeruginosa ZJB1125 harboring a stereoselective 2-hydroxyacid dehydrogenase (2-HADH) can catalyze asymmetric oxidation of mandelic acid and 2-chloromandelic acid into (R)-isomers and corresponding 2-ketoacids with high activity and enantioselectivity, while no consecutive oxidation of 2-ketoacids was observed during whole-cell catalysis. The 2-HADH in P. aeruginosa ZJB1125 is a FMN-dependent flavoprotein and did not require NAD(P)⺠as cofactors to catalyze the oxidation reaction. Enzyme activity staining identified 2-HADH as the key enzyme that enantioselectively oxidized (S)-hydroxyacid to 2-ketoacid. The 2-HADH in P. aeruginosa ZJB1125 is inducible and 2-chloromandelic acid was found to induce its synthesis efficiently. The bacterium displayed pretty high activity and enantioselectivity for most of the aromatic 2-hydroxyacids examined, and have a potential for the concurrent obtaining of aromatic (R)-2-hydroxyacids and aromatic 2-ketoacids in near theoretical conversions. Using a simple organic extract process, aromatic (R)-2-hydroxyacids and aromatic 2-ketoacids can be effectively separated from the biocatalytic reaction mixture with high yield (>95%). This work provided a novel method for the concurrent obtaining of aromatic (R)-2-hydroxyacids and aromatic 2-ketoacids by oxidation of aromatic 2-hydroxyacids in one-step biotransformation, which would be a valuable process due to its high atom economy.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Pseudomonas aeruginosa
/
Cetoácidos
/
Ácidos Mandélicos
Idioma:
En
Revista:
J Biotechnol
Assunto da revista:
BIOTECNOLOGIA
Ano de publicação:
2013
Tipo de documento:
Article
País de afiliação:
China