Quantification of biomass and cell motion in human pluripotent stem cell colonies.
Biophys J
; 105(3): 593-601, 2013 Aug 06.
Article
em En
| MEDLINE
| ID: mdl-23931307
ABSTRACT
Somatic cell reprogramming to pluripotency requires an immediate increase in cell proliferation and reduction in cell size. It is unknown whether proliferation and biomass controls are similarly coordinated with early events during the differentiation of pluripotent stem cells (PSCs). This impasse exists because PSCs grow in tight clusters or colonies, precluding most quantifying approaches. Here, we investigate live cell interferometry as an approach to quantify the biomass and growth of HSF1 human PSC colonies before and during retinoic acid-induced differentiation. We also provide an approach for measuring the rate and coordination of intracolony mass redistribution in HSF1 clusters using live cell interferometry images. We show that HSF1 cells grow at a consistent, exponential rate regardless of colony size and display coordinated intracolony movement that ceases with the onset of differentiation. By contrast, growth and proliferation rates show a decrease of only â¼15% decrease during early differentiation despite global changes in gene expression and previously reported changes in energy metabolism. Overall, these results suggest that cell biomass and proliferation are regulated independent of pluripotency during early differentiation, which is distinct from what occurs with successful reprogramming.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Movimento Celular
/
Biomassa
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Células-Tronco Pluripotentes
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Células-Tronco Embrionárias
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Corpos Embrioides
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Interferometria
Limite:
Humans
Idioma:
En
Revista:
Biophys J
Ano de publicação:
2013
Tipo de documento:
Article
País de afiliação:
Estados Unidos