Cyclosporine A at reperfusion fails to reduce infarct size in the in vivo rat heart.
Basic Res Cardiol
; 108(5): 379, 2013 Sep.
Article
em En
| MEDLINE
| ID: mdl-23955512
ABSTRACT
We examined the effects on infarct size and mitochondrial function of ischemic (Isch), cyclosporine A (CsA) and isoflurane (Iso) preconditioning and postconditioning in the in vivo rat model. Anesthetized open-chest rats underwent 30 min of ischemia followed by either 120 min (protocol 1 infarct size assessment) or 15 min of reperfusion (protocol 2 assessment of mitochondrial function). All treatments administered before the 30-min ischemia (Pre-Isch, Pre-CsA, Pre-Iso) significantly reduced infarct as compared to control. In contrast, only Post-Iso significantly reduced infarct size, while Post-Isch and Post-CsA had no significant protective effect. As for the postconditioning-like interventions, the mitochondrial calcium retention capacity significantly increased only in the Post-Iso group (+58 % vs control) after succinate activation. Only Post-Iso increased state 3 (+177 and +62 %, for G/M and succinate, respectively) when compared to control. Also, Post-Iso reduced the hydrogen peroxide (H2O2) production (-46 % vs control) after complex I activation. This study suggests that isoflurane, but not cyclosporine A, can prevent lethal reperfusion injury in this in vivo rat model. This might be related to the need for a combined effect on cyclophilin D and complex I during the first minutes of reperfusion.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Traumatismo por Reperfusão Miocárdica
/
Ciclosporina
/
Inibidores Enzimáticos
/
Infarto do Miocárdio
Tipo de estudo:
Guideline
Limite:
Animals
Idioma:
En
Revista:
Basic Res Cardiol
Ano de publicação:
2013
Tipo de documento:
Article
País de afiliação:
França