Crystallization and preliminary X-ray diffraction studies of the reduced form of the terminal oxygenase component of the Rieske nonhaem iron oxygenase system carbazole 1,9a-dioxygenase.
Acta Crystallogr Sect F Struct Biol Cryst Commun
; 69(Pt 11): 1284-7, 2013 Nov.
Article
em En
| MEDLINE
| ID: mdl-24192370
ABSTRACT
The initial reaction of bacterial carbazole degradation is catalysed by carbazole 1,9a-dioxygenase, which consists of terminal oxygenase, ferredoxin and ferredoxin reductase components. The reduced form of the terminal oxygenase component was crystallized at 293â
K by the hanging-drop vapour-diffusion method using PEG MME 550 as the precipitant under anaerobic conditions. The crystals diffracted to a resolution of 1.74â
Å and belonged to space group P6(5), with unit-cell parameters a = b = 92.0, c = 243.6â
Å. The asymmetric unit contained a trimer of terminal oxygenase molecules.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas de Bactérias
/
Complexo III da Cadeia de Transporte de Elétrons
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Proteobactérias
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Dioxigenases
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Ferro
Idioma:
En
Revista:
Acta Crystallogr Sect F Struct Biol Cryst Commun
Ano de publicação:
2013
Tipo de documento:
Article
País de afiliação:
Japão