Plasmid-mediated transformation tropism of chlamydial biovars.
Pathog Dis
; 70(2): 189-93, 2014 Mar.
Article
em En
| MEDLINE
| ID: mdl-24214488
ABSTRACT
Chlamydia trachomatis and C. muridarum are human and mouse pathogens, respectively, which show high conservation of gene order and content. Both species contain a common 7.5-kb plasmid that is an important virulence factor. Recently described transformation systems have been used to characterize C. trachomatis L2 plasmid gene functions; however, similar studies have not been reported for C. trachomatis ocular tropic serovar A or the mouse strain, C. muridarum. Here, we have conducted genetic experiments with C. trachomatis serovar A and C. muridarum and report the following (1) successful transformation of C. muridarum and C. trachomatis serovar A is restricted to a shuttle vector with a C. muridarum or C. trachomatis serovar A plasmid backbone, respectively; (2) transformation of plasmid-deficient C. muridarum with the C. muridarum-based shuttle vector complement glycogen accumulation and inclusion morphology; and (3) C. muridarum plasmid-encoded Pgp4 is a regulator of chromosomal (glgA) and plasmid (pgp3) virulence genes. In summary, our findings show a previously unrecognized and unexpected role for the chlamydial plasmid in its transformation tropism and confirm the plasmids regulatory role of virulence genes in C. muridarum.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Plasmídeos
/
Transformação Bacteriana
/
Chlamydia trachomatis
/
Chlamydia muridarum
/
Transferência Genética Horizontal
/
Especificidade de Hospedeiro
Limite:
Animals
/
Humans
Idioma:
En
Revista:
Pathog Dis
Ano de publicação:
2014
Tipo de documento:
Article
País de afiliação:
China