Optimization for production of exopolysaccharides with antitumor activity in vitro from Paecilomyces hepiali.
Carbohydr Polym
; 99: 226-34, 2014 Jan.
Article
em En
| MEDLINE
| ID: mdl-24274500
ABSTRACT
In the present study, optimal medium for the growth of mycelia and the production of exopolysaccharides from Paecilomyces hepiali HN1 (PHEPS) in submerged culture was investigated. As a result, the maximum production of mycelia (12.98 ± 0.14 g/L) and PHEPS (5.33 ± 0.11 g/L) were achieved under the optimal medium of sucrose 46.08 g/L, yeast extract 4.71 g/L, (NH4)2SO4 5.72 g/L, KH2PO4 1.70 g/L, CaCl2 0.50 g/L, MgSO4 0.50 g/L, potato extract 1% and malt extract 1%. Furthermore, the antitumor activity of PHEPS in vitro was evaluated by using three cell lines of human liver tumor HepG2 cells, breast cancer MCF-7 cells and cervical cancer Hela cells. It was found that PHEPS exhibited relative higher anti-proliferative activity against HepG2 cells than MCF-7 cells and Hela cells. At a concentration of 500 µg/mL and 72 h treatment, the inhibition rate of PHEPS on HepG2 cells reached to 62.58%. All these results suggested that PHEPS could be explored as novel natural antitumor agent with great potential application.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Paecilomyces
/
Micélio
/
Polissacarídeos Fúngicos
/
Antineoplásicos
Limite:
Female
/
Humans
Idioma:
En
Revista:
Carbohydr Polym
Ano de publicação:
2014
Tipo de documento:
Article