Gut adhesive Bacillus subtilis spores as a platform for mucosal delivery of antigens.
Infect Immun
; 82(4): 1414-23, 2014 Apr.
Article
em En
| MEDLINE
| ID: mdl-24421038
ABSTRACT
Bacillus subtilis spores have been used as safe and heat-resistant antigen delivery vectors. Nonetheless, the oral administration of spores typically induces weak immune responses to the passenger antigens, which may be attributed to the fast transit through the gastrointestinal tract. To overcome this limitation, we have developed B. subtilis spores capable of binding to the gut epithelium by means of expressing bacterial adhesins on the spore surface. The resulting spores bound to in vitro intestinal cells, showed a longer transit through the mouse intestinal tract, and interacted with Peyer's patch cells. The adhesive spores increased the systemic and secreted antibody responses to the Streptococcus mutans P1 protein, used as a model antigen, following oral, intranasal, and sublingual administration. Additionally, P1-specific antibodies efficiently inhibited the adhesion of the oral pathogen Streptococcus mutans to abiotic surfaces. These results support the use of gut-colonizing B. subtilis spores as a new platform for the mucosal delivery of vaccine antigens.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Esporos Bacterianos
/
Bacillus subtilis
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Vacinas Bacterianas
/
Mucosa Gástrica
/
Antígenos de Bactérias
Limite:
Animals
Idioma:
En
Revista:
Infect Immun
Ano de publicação:
2014
Tipo de documento:
Article
País de afiliação:
Brasil