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[RIN1 expression in hepatocellular cancer and the affection on prognosis and tumor invasion ability].
Liu, Hai-yang; Wu, Gang; He, Hui; Wang, Ya-wei; Cheng, Ying; Liu, Yong-feng.
Afiliação
  • Liu HY; Department of Hepatobiliary Surgery, Division of General Surgery, the First Hospital of China Medical University, Shenyang 110001, China.
  • Wu G; Department of Hepatobiliary Surgery, Division of General Surgery, the First Hospital of China Medical University, Shenyang 110001, China. Email: wgzwl@hotmail.com.
  • He H; Department of Hepatobiliary Surgery, Division of General Surgery, the First Hospital of China Medical University, Shenyang 110001, China.
  • Wang YW; Department of Hepatobiliary Surgery, Division of General Surgery, the First Hospital of China Medical University, Shenyang 110001, China.
  • Cheng Y; Department of Hepatobiliary Surgery, Division of General Surgery, the First Hospital of China Medical University, Shenyang 110001, China.
  • Liu YF; Department of Hepatobiliary Surgery, Division of General Surgery, the First Hospital of China Medical University, Shenyang 110001, China.
Zhonghua Wai Ke Za Zhi ; 51(11): 1025-9, 2013 Nov.
Article em Zh | MEDLINE | ID: mdl-24444690
OBJECTIVE: To test the expression of RIN1 in hepatocellular cancer (HCC) and study its clinicopathological significance and mechanism. METHODS: RIN1 mRNA in 36 HCC tissues was analyzed using real-time PCR (RT-PCR). The expression of RIN1 was examined by immunohistochemistry (IHC) in 110 HCC specimens. The relationship between the protein expression and prognosis was analyzed. Transwell was used to test invasion ability of HCC cell lines which were transfected with the expression vector pEGFP-N1-RIN1. RESULTS: RIN1 mRNA expression levels was much lower in tumor tissues than that in their corresponding non-cancerous tissues (χ(2) = 7.430, P = 0.026). RIN1 protein was lowly expressed in liver cancer samples (69.1%) and correlated with poor survival (6.46%) (χ(2) = 13.808, P < 0.05). Transwell assays show that RIN1 overexpression can inhibit invasion ability of HepG2 cells (t = 8.975 and 9.522, both P < 0.05). RIN1 expression and ABL2 and E calcium protein were positively correlated (r = 0.898 and 0.912, P < 0.05), and negatively correlated with MMP-9 (r = -0.933, P = 0.002). CONCLUSIONS: RIN1 expression was down-regulated in HCC and low expression of RIN1 foreshows poor prognosis of HCC patients. RIN1 overexpression can inhibit invasion ability of HepG2 cells.
Assuntos
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Carcinoma Hepatocelular / Peptídeos e Proteínas de Sinalização Intracelular / Neoplasias Hepáticas Tipo de estudo: Prognostic_studies Limite: Adult / Aged / Female / Humans / Middle aged Idioma: Zh Revista: Zhonghua Wai Ke Za Zhi Ano de publicação: 2013 Tipo de documento: Article País de afiliação: China
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Carcinoma Hepatocelular / Peptídeos e Proteínas de Sinalização Intracelular / Neoplasias Hepáticas Tipo de estudo: Prognostic_studies Limite: Adult / Aged / Female / Humans / Middle aged Idioma: Zh Revista: Zhonghua Wai Ke Za Zhi Ano de publicação: 2013 Tipo de documento: Article País de afiliação: China