BurrH: a new modular DNA binding protein for genome engineering.
Sci Rep
; 4: 3831, 2014 Jan 23.
Article
em En
| MEDLINE
| ID: mdl-24452192
ABSTRACT
The last few years have seen the increasing development of new DNA targeting molecular tools and strategies for precise genome editing. However, opportunities subsist to either improve or expand the current toolbox and further broaden the scope of possible biotechnological applications. Here we report the discovery and the characterization of BurrH, a new modular DNA binding protein from Burkholderia rhizoxinica that is composed of highly polymorphic DNA targeting modules. We also engineered this scaffold to create a new class of designer nucleases that can be used to efficiently induce in vivo targeted mutagenesis and targeted gene insertion at a desired locus.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Engenharia Genética
/
Genoma Humano
/
Mutagênese Insercional
/
Burkholderia
/
Proteínas de Ligação a DNA
Limite:
Humans
Idioma:
En
Revista:
Sci Rep
Ano de publicação:
2014
Tipo de documento:
Article