ngsTools: methods for population genetics analyses from next-generation sequencing data.
Bioinformatics
; 30(10): 1486-7, 2014 May 15.
Article
em En
| MEDLINE
| ID: mdl-24458950
ABSTRACT
SUMMARY:
Next-generation sequencing technologies produce short reads that are either de novo assembled or mapped to a reference genome. Genotypes and/or single-nucleotide polymorphisms are then determined from the read composition at each site, which become the basis for many downstream analyses. However, for low sequencing depths, e.g. , there is considerable statistical uncertainty in the assignment of genotypes because of random sampling of homologous base pairs in heterozygotes and sequencing or alignment errors. Recently, several probabilistic methods have been proposed to account for this uncertainty and make accurate inferences from low quality and/or coverage sequencing data. We present ngsTools, a collection of programs to perform population genetics analyses from next-generation sequencing data. The methods implemented in these programs do not rely on single-nucleotide polymorphism or genotype calling and are particularly suitable for low sequencing depth data.AVAILABILITY:
Programs included in ngsTools are implemented in C/C++ and are freely available for noncommercial use at https//github.com/mfumagalli/ngsTools. CONTACT mfumagalli82@gmail.com SUPPLEMENTARY INFORMATION Supplementary materials are available at Bioinformatics online.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Sequenciamento de Nucleotídeos em Larga Escala
Idioma:
En
Revista:
Bioinformatics
Assunto da revista:
INFORMATICA MEDICA
Ano de publicação:
2014
Tipo de documento:
Article
País de afiliação:
Dinamarca