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Efficient genome modification by CRISPR-Cas9 nickase with minimal off-target effects.
Shen, Bin; Zhang, Wensheng; Zhang, Jun; Zhou, Jiankui; Wang, Jianying; Chen, Li; Wang, Lu; Hodgkins, Alex; Iyer, Vivek; Huang, Xingxu; Skarnes, William C.
Afiliação
  • Shen B; 1] Ministry of Education Key Laboratory of Model Animal for Disease Study, Model Animal Research Center of Nanjing University, Nanjing, China. [2].
  • Zhang W; 1] Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, UK. [2].
  • Zhang J; 1] Ministry of Education Key Laboratory of Model Animal for Disease Study, Model Animal Research Center of Nanjing University, Nanjing, China. [2].
  • Zhou J; Ministry of Education Key Laboratory of Model Animal for Disease Study, Model Animal Research Center of Nanjing University, Nanjing, China.
  • Wang J; Ministry of Education Key Laboratory of Model Animal for Disease Study, Model Animal Research Center of Nanjing University, Nanjing, China.
  • Chen L; Ministry of Education Key Laboratory of Model Animal for Disease Study, Model Animal Research Center of Nanjing University, Nanjing, China.
  • Wang L; Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing, China.
  • Hodgkins A; Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, UK.
  • Iyer V; Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, UK.
  • Huang X; Ministry of Education Key Laboratory of Model Animal for Disease Study, Model Animal Research Center of Nanjing University, Nanjing, China.
  • Skarnes WC; Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, UK.
Nat Methods ; 11(4): 399-402, 2014 Apr.
Article em En | MEDLINE | ID: mdl-24584192
ABSTRACT
Bacterial RNA-directed Cas9 endonuclease is a versatile tool for site-specific genome modification in eukaryotes. Co-microinjection of mouse embryos with Cas9 mRNA and single guide RNAs induces on-target and off-target mutations that are transmissible to offspring. However, Cas9 nickase can be used to efficiently mutate genes without detectable damage at known off-target sites. This method is applicable for genome editing of any model organism and minimizes confounding problems of off-target mutations.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Genoma Bacteriano / Desoxirribonuclease I / Endonucleases / Sistemas CRISPR-Cas Limite: Animals / Humans Idioma: En Revista: Nat Methods Assunto da revista: TECNICAS E PROCEDIMENTOS DE LABORATORIO Ano de publicação: 2014 Tipo de documento: Article
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Genoma Bacteriano / Desoxirribonuclease I / Endonucleases / Sistemas CRISPR-Cas Limite: Animals / Humans Idioma: En Revista: Nat Methods Assunto da revista: TECNICAS E PROCEDIMENTOS DE LABORATORIO Ano de publicação: 2014 Tipo de documento: Article