Direct and single-molecule visualization of the solution-state structures of G-hairpin and G-triplex intermediates.
Angew Chem Int Ed Engl
; 53(16): 4107-12, 2014 Apr 14.
Article
em En
| MEDLINE
| ID: mdl-24623581
ABSTRACT
We present the direct and single-molecule visualization of the in-pathway intermediates of the G-quadruplex folding that have been inaccessible by any experimental method employed to date. Using DNA origami as a novel tool for the structural control and high-speed atomic force microscopy (HS-AFM) for direct visualization, we captured images of the unprecedented solution-state structures of a tetramolecular antiparallel and (3+1)-type G-quadruplex intermediates, such as G-hairpin and G-triplex, with nanometer precision. No such structural information was reported previously with any direct or indirect technique, solution or solid-state, single-molecule or bulk studies, and at any resolution. Based on our results, we proposed a folding mechanism of these G-quadruplexes.
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1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
DNA
/
Microscopia de Força Atômica
/
Quadruplex G
Idioma:
En
Revista:
Angew Chem Int Ed Engl
Ano de publicação:
2014
Tipo de documento:
Article