Recognition of oligonucleotide-encoded T cell epitopes introduced into a gene unrelated to the original antigen.
J Exp Med
; 169(1): 297-302, 1989 Jan 01.
Article
em En
| MEDLINE
| ID: mdl-2462610
ABSTRACT
We have previously demonstrated that H-2Kd-restricted CTL specific for HLA-CW3 or HLA-A24 can recognize synthetic peptides corresponding to residues 170-182 of the HLA molecules. Synthetic oligonucleotides encoding region 170-182 of CW3 or A24 were inserted into the influenza nucleoprotein (NP) gene. We demonstrate herein that P815 (H-2d) cells transfected with the NP-oligo recombinant genes are specifically lysed by HLA-specific Kd-restricted CTL clones. Our results imply that there must be a high degree of flexibility for the expression of T cell epitopes in different molecular contexts.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas Virais
/
Proteínas Recombinantes de Fusão
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Proteínas Recombinantes
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Linfócitos T
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Proteínas do Core Viral
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Proteínas de Ligação a RNA
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Antígenos HLA
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Nucleoproteínas
Limite:
Animals
/
Humans
Idioma:
En
Revista:
J Exp Med
Ano de publicação:
1989
Tipo de documento:
Article
País de afiliação:
França