Multifluorescence live analysis of herpes simplex virus type-1 replication.

Seyffert, Michael; de Oliveira, Anna Paula; Fraefel, Cornel; Vogel, Rebecca

Seyffert, Michael; de Oliveira, Anna Paula; Fraefel, Cornel; Vogel, Rebecca.

Afiliação

Seyffert M; Institute of Virology, University of Zurich, Winterthurerstr. 266a, 8057, Zurich, Switzerland, mseyffert@vetvir.uzh.ch.

Methods Mol Biol ; 1144: 235-47, 2014.

Article em En | MEDLINE | ID: mdl-24671688

ABSTRACT

ABSTRACT

The possibility to label specific viral and cellular structures with live cell markers such as autofluorescent proteins has greatly contributed to our understanding of diverse steps of the virus life cycle, as it allows monitoring virus replication in a spatial and temporal fashion. Here, we describe the multi-fluorescent analysis of the multi-compartment herpes simplex virus type-1 by live-cell confocal laser scanning microscopy.

Assuntos

Herpesvirus Humano 1/genética; Biologia Molecular/métodos; Replicação Viral/genética; Animais; Linhagem Celular; Chlorocebus aethiops; Herpesvirus Humano 1/metabolismo; Herpesvirus Humano 1/ultraestrutura; Humanos; Microscopia Confocal; Células Vero

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Replicação Viral / Herpesvirus Humano 1 / Biologia Molecular Limite: Animals / Humans Idioma: En Revista: Methods Mol Biol Assunto da revista: BIOLOGIA MOLECULAR Ano de publicação: 2014 Tipo de documento: Article

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