Cyclosporin A inhibits directly in vivo keratinocyte proliferation of living human skin.
J Invest Dermatol
; 92(5): 755-7, 1989 May.
Article
em En
| MEDLINE
| ID: mdl-2469739
ABSTRACT
A direct in vivo antiproliferative effect of cyclosporin A (CsA) on human epidermal keratinocytes (EK) grafted onto nude mice was evaluated. Using pulse-labeling of 5-bromo-2'-deoxyuridine (BrdU), a thymidine analogue incorporated into the nuclei of DNA-synthesizing (S-phase) cells, the antiproliferative effect of CsA was revealed as a decrease in the number of BrdU-positive human EK grafted onto nude mice receiving a daily subcutaneous injection of 50 mg/kg of CsA. The blood level of CsA in the treated mice, evaluated by a radioimmunologic assay, was 679 +/- 501 ng/ml (n = 3). Using an antibody to leukocyte common antigen, it was shown that no human lymphocytes were present in the grafted skin. Therefore, this antiproliferative effect of CsA on human EK seems to be due to a direct effect on EK rather than to lymphocyte regulation.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Pele
/
Ciclosporinas
/
Queratinas
Limite:
Animals
/
Female
/
Humans
Idioma:
En
Revista:
J Invest Dermatol
Ano de publicação:
1989
Tipo de documento:
Article
País de afiliação:
França