CTSB promotes porcine preadipocytes differentiation by degrading fibronectin and attenuating the Wnt/ß-catenin signaling pathway.
Mol Cell Biochem
; 395(1-2): 53-64, 2014 Oct.
Article
em En
| MEDLINE
| ID: mdl-24878992
ABSTRACT
The process of preadipocytes differentiation plays a vital role in adipose tissue expansion and many factors are involved in this event. Cathepsin B (CTSB), secreted from lysosome, has been reported in regulating a variety of physiological processes. In this study, we demonstrated CTSB promotes lipid accumulation and adipogenic genes expression in porcine primary preadipocytes by degrading fibronectin (Fn), a key component of extracellular matrix. Lithium chloride (LiCl) is an activator of Wnt/ß-catenin signaling through stabilizing ß-catenin. We found that CTSB can relieve the anti-adipogenic effects of LiCl, indicating that CTSB could impact Wnt/ß-catenin signaling pathway. Interestingly, Fn is an important target gene of Wnt/ß-catenin. So we considered that CTSB promote preadipocytes differentiation by suppressing these two pathways.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Catepsina B
/
Fibronectinas
/
Cloreto de Lítio
/
Adipócitos
/
Matriz Extracelular
Limite:
Animals
Idioma:
En
Revista:
Mol Cell Biochem
Ano de publicação:
2014
Tipo de documento:
Article