Human cardiac myosin heavy chain gene mapped within chromosome region 14q11.2----q13.
Am J Med Genet
; 32(2): 279-84, 1989 Feb.
Article
em En
| MEDLINE
| ID: mdl-2494889
ABSTRACT
Our previous report demonstrated that two human cardiac alpha- and beta-myosin heavy-chains (MHCs) which correspond to MYH6 and MYH7 respectively, according to Human Gene Mapping nomenclature, were mapped to human chromosome 14 and that human cardiac and skeletal MHC genes do not cosegregate. For further analysis, the regional mapping method was used. DNA from 4 human deletion and 3 human duplication cell lines were prepared for southern blotting, hybridized with human cardiac alpha- and beta-MHC DNA probes, and the hybridization intensity relative to 46,XX or 46,XY DNA was estimated. The results showed that two human cardiac MHC genes segregated with the 14cen----q13 region of the long arm of human chromosome 14. In situ hybridization of 3H-labeled human cardiac alpha-MHC probe to normal human metaphase chromosome independently confirmed this result.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Cromossomos Humanos Par 14
/
Mapeamento Cromossômico
/
Miosinas
/
Cadeias Pesadas de Imunoglobulinas
/
Miocárdio
Limite:
Humans
Idioma:
En
Revista:
Am J Med Genet
Ano de publicação:
1989
Tipo de documento:
Article