Characterization of the interaction between lysyl-tRNA synthetase and laminin receptor by NMR.
FEBS Lett
; 588(17): 2851-8, 2014 Aug 25.
Article
em En
| MEDLINE
| ID: mdl-24983501
ABSTRACT
Lysyl-tRNA synthetase (KRS) interacts with the laminin receptor (LR/RPSA) and enhances laminin-induced cell migration in cancer metastasis. In this nuclear magnetic resonance (NMR)-based study, we show that the anticodon-binding domain of KRS binds directly to the C-terminal region of 37LRP, and the previously found inhibitors BC-K-01 and BC-K-YH16899 interfere with KRS-37LRP binding. In addition, the anticodon-binding domain of KRS binds to laminin, observed by NMR and SPR. These results provide crucial insights into the structural characteristics of the KRS-LR interaction on the cell surface.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Receptores de Laminina
/
Ressonância Magnética Nuclear Biomolecular
/
Lisina-tRNA Ligase
Tipo de estudo:
Prognostic_studies
Limite:
Humans
Idioma:
En
Revista:
FEBS Lett
Ano de publicação:
2014
Tipo de documento:
Article