Vaccine focusing to cross-subtype HIV-1 gp120 variable loop epitopes.
Vaccine
; 32(39): 4916-24, 2014 Sep 03.
Article
em En
| MEDLINE
| ID: mdl-25045827
ABSTRACT
We designed synthetic, epitope-focused immunogens that preferentially display individual neutralization epitopes targeted by cross-subtype anti-HIV V3 loop neutralizing monoclonal antibodies (mAbs). Vaccination of rabbits with these immunogens resulted in the elicitation of distinct polyclonal serum Abs that exhibit cross-subtype neutralization specificities mimicking the mAbs that guided the design. Our results prove the principle that a predictable range of epitope-specific polyclonal cross-subtype HIV-1 neutralizing Abs can be intentionally elicited in mammals by vaccination. The precise boundaries of the epitopes and conformational flexibility in the presentation of the epitopes in the immunogen appeared to be important for successful elicitation. This work may serve as a starting point for translating the activities of human broadly neutralizing anti-HIV-1 monoclonal antibodies (bNAbs) into matched immunogens that can contribute to an efficacious HIV-1 vaccine.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Anticorpos Anti-HIV
/
Proteína gp120 do Envelope de HIV
/
Vacinas contra a AIDS
/
Epitopos
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
En
Revista:
Vaccine
Ano de publicação:
2014
Tipo de documento:
Article