Your browser doesn't support javascript.
loading
Direct silylation of Trypanosoma brucei metabolites in aqueous samples and their GC-MS/MS analysis.
Podolec, Peter; Szabó, Alexandra Hengerics; Blasko, Jaroslav; Kubinec, Róbert; Górová, Renáta; Visnovský, Jozef; Gnipová, Anna; Horváth, Anton; Bierhanzl, Václav; Hlozek, Tomás; Cabala, Radomír.
Afiliação
  • Podolec P; Institute of Chemistry, Faculty of Natural Sciences, Comenius University, Mlynská dolina CH-2, 842 15 Bratislava, Slovakia.
  • Szabó AH; Institute of Chemistry, Faculty of Natural Sciences, Comenius University, Mlynská dolina CH-2, 842 15 Bratislava, Slovakia.
  • Blasko J; Institute of Chemistry, Faculty of Natural Sciences, Comenius University, Mlynská dolina CH-2, 842 15 Bratislava, Slovakia.
  • Kubinec R; Institute of Chemistry, Faculty of Natural Sciences, Comenius University, Mlynská dolina CH-2, 842 15 Bratislava, Slovakia. Electronic address: kubinec@fns.uniba.sk.
  • Górová R; Institute of Chemistry, Faculty of Natural Sciences, Comenius University, Mlynská dolina CH-2, 842 15 Bratislava, Slovakia.
  • Visnovský J; Institute of Chemistry, Faculty of Natural Sciences, Comenius University, Mlynská dolina CH-2, 842 15 Bratislava, Slovakia; SynthCluster, s.r.o., Komenského 1439, 900 01 Modra, Slovakia.
  • Gnipová A; Department of Biochemistry, Faculty of Natural Sciences, Comenius University, Mlynská dolina CH-1, 842 15 Bratislava, Slovakia.
  • Horváth A; Department of Biochemistry, Faculty of Natural Sciences, Comenius University, Mlynská dolina CH-1, 842 15 Bratislava, Slovakia.
  • Bierhanzl V; Department of Analytical Chemistry, Faculty of Science, Charles University in Prague, Albertov 6, 128 43 Prague 2, Czech Republic.
  • Hlozek T; Institute of Forensic Medicine and Toxicology, General University Hospital in Prague, U Nemocnice 2, 128 08 Prague 2, Czech Republic.
  • Cabala R; Department of Analytical Chemistry, Faculty of Science, Charles University in Prague, Albertov 6, 128 43 Prague 2, Czech Republic; Institute of Forensic Medicine and Toxicology, General University Hospital in Prague, U Nemocnice 2, 128 08 Prague 2, Czech Republic.
J Chromatogr B Analyt Technol Biomed Life Sci ; 967: 134-8, 2014 Sep 15.
Article em En | MEDLINE | ID: mdl-25089958
ABSTRACT
A simple two-step method for the derivatization of polar compounds (lactate, alanine, glycerol, succinate and glucose) using hexamethyldisilazane (HMDS) and N,O-bis(trimethylsilyl)trifluoroacetamide (BSTFA) was developed. This method allows direct derivatization of aqueous samples wihout sample pretreatment. The method was used for the analysis of the metabolites of the unicellular organism Trypanosoma brucei. The limits of detection by GC-MS/MS analysis were in the range of 0.02 mg L(-1) for glucose to 0.85 mg L(-1) for lactate.
Assuntos
Palavras-chave
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Trypanosoma brucei brucei / Cromatografia Gasosa-Espectrometria de Massas Idioma: En Revista: J Chromatogr B Analyt Technol Biomed Life Sci Assunto da revista: ENGENHARIA BIOMEDICA Ano de publicação: 2014 Tipo de documento: Article País de afiliação: Eslováquia
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Trypanosoma brucei brucei / Cromatografia Gasosa-Espectrometria de Massas Idioma: En Revista: J Chromatogr B Analyt Technol Biomed Life Sci Assunto da revista: ENGENHARIA BIOMEDICA Ano de publicação: 2014 Tipo de documento: Article País de afiliação: Eslováquia