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Phenotypic detection of carbapenemase-producing Enterobacteriaceae by use of matrix-assisted laser desorption ionization-time of flight mass spectrometry and the Carba NP test.
Knox, James; Jadhav, Snehal; Sevior, Danielle; Agyekum, Alex; Whipp, Margaret; Waring, Lynette; Iredell, Jonathan; Palombo, Enzo.
Afiliação
  • Knox J; Microbiology Department, Melbourne Pathology, Melbourne, Australia jaknox2@yahoo.com.au.
  • Jadhav S; Department of Chemistry and Biotechnology, Swinburne University of Technology, Melbourne, Australia.
  • Sevior D; bioMérieux Australia Pty Ltd., Sydney, Australia.
  • Agyekum A; Westmead Millennium and Marie Bashir Institutes, University of Sydney, Sydney, Australia.
  • Whipp M; Microbiological Diagnostic Unit, Public Health Laboratory, University of Melbourne, Melbourne, Australia.
  • Waring L; Microbiology Department, Melbourne Pathology, Melbourne, Australia.
  • Iredell J; Westmead Millennium and Marie Bashir Institutes, University of Sydney, Sydney, Australia Centre for Infectious Diseases and Microbiology, Westmead Hospital, Westmead, Australia.
  • Palombo E; Department of Chemistry and Biotechnology, Swinburne University of Technology, Melbourne, Australia.
J Clin Microbiol ; 52(11): 4075-7, 2014 Nov.
Article em En | MEDLINE | ID: mdl-25187633
ABSTRACT
We compared the diagnostic accuracy of the Carba NP test with that of a straightforward matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) method for detecting carbapenemase-producing Enterobacteriaceae (CPE). Using PCR as the reference method, both tests demonstrated a sensitivity of 87% and a specificity of 100%. MALDI-TOF MS offers a potential alternative for the rapid detection of CPE in the clinical laboratory setting.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proteínas de Bactérias / Beta-Lactamases / Técnicas Bacteriológicas / Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz / Enterobacteriaceae Tipo de estudo: Diagnostic_studies / Evaluation_studies Limite: Humans Idioma: En Revista: J Clin Microbiol Ano de publicação: 2014 Tipo de documento: Article País de afiliação: Austrália
Texto completo
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PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proteínas de Bactérias / Beta-Lactamases / Técnicas Bacteriológicas / Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz / Enterobacteriaceae Tipo de estudo: Diagnostic_studies / Evaluation_studies Limite: Humans Idioma: En Revista: J Clin Microbiol Ano de publicação: 2014 Tipo de documento: Article País de afiliação: Austrália