Effects of acute doxorubicin treatment on hepatic proteome lysine acetylation status and the apoptotic environment.
World J Biol Chem
; 5(3): 377-86, 2014 Aug 26.
Article
em En
| MEDLINE
| ID: mdl-25225604
ABSTRACT
AIM:
To determine if doxorubicin (Dox) alters hepatic proteome acetylation status and if acetylation status was associated with an apoptotic environment.METHODS:
Doxorubicin (20 mg/kg; Sigma, Saint Louis, MO; n = 8) or NaCl (0.9%; n = 7) was administered as an intraperitoneal injection to male F344 rats, 6-wk of age. Once animals were treated with Dox or saline, all animals were fasted until sacrifice 24 h later.RESULTS:
Dox treatment decreased proteome lysine acetylation likely due to a decrease in histone acetyltransferase activity. Proteome deacetylation may likely not be associated with a proapoptotic environment. Dox did not increase caspase-9, -8, or -3 activation nor poly (adenosine diphosphate-ribose) polymerase-1 cleavage. Dox did stimulate caspase-12 activation, however, it likely did not play a role in apoptosis induction.CONCLUSION:
Early effects of Dox involve hepatic proteome lysine deacetylation and caspase-12 activation under these experimental conditions.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Idioma:
En
Revista:
World J Biol Chem
Ano de publicação:
2014
Tipo de documento:
Article
País de afiliação:
Estados Unidos