Dyskerin, tRNA genes, and condensin tether pericentric chromatin to the spindle axis in mitosis.
J Cell Biol
; 207(2): 189-99, 2014 Oct 27.
Article
em En
| MEDLINE
| ID: mdl-25332162
ABSTRACT
Condensin is enriched in the pericentromere of budding yeast chromosomes where it is constrained to the spindle axis in metaphase. Pericentric condensin contributes to chromatin compaction, resistance to microtubule-based spindle forces, and spindle length and variance regulation. Condensin is clustered along the spindle axis in a heterogeneous fashion. We demonstrate that pericentric enrichment of condensin is mediated by interactions with transfer ribonucleic acid (tRNA) genes and their regulatory factors. This recruitment is important for generating axial tension on the pericentromere and coordinating movement between pericentromeres from different chromosomes. The interaction between condensin and tRNA genes in the pericentromere reveals a feature of yeast centromeres that has profound implications for the function and evolution of mitotic segregation mechanisms.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Saccharomyces cerevisiae
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RNA de Transferência
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Cromatina
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Ribonucleoproteínas Nucleares Pequenas
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Adenosina Trifosfatases
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Proteínas de Saccharomyces cerevisiae
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Complexos Multiproteicos
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Proteínas de Ligação a DNA
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Hidroliases
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Proteínas Associadas aos Microtúbulos
Idioma:
En
Revista:
J Cell Biol
Ano de publicação:
2014
Tipo de documento:
Article