Human telomerase reverse transcriptase is a promising target for cancer inhibition in squamous cell carcinomas.
Anticancer Res
; 34(11): 6389-95, 2014 Nov.
Article
em En
| MEDLINE
| ID: mdl-25368238
ABSTRACT
BACKGROUND/AIM:
The present study aimed to investigate whether the down-regulation of human telomerase reverse transcriptase (hTERT) may induce an anti-invasive effect in oral squamous cell cancer cell lines. MATERIALS ANDMETHODS:
A genetically-engineered squamous carcinoma cell line overexpressing hTERT in immortalized oral keratinocytes transfected by human papilloma virus (HPV)-16 E6/E7 (IHOK) was used. In vivo tumorigenicity was examined using an orthotopic xenograft model of nude mice. For evaluating anti-invasive activity by knockdown of hTERT expression, transwell invasion assay and real-time polymerase chain reaction (PCR) for matrix metalloproteinases (MMP) were employed.RESULTS:
The down-regulation of hTERT expression reduced the invasive activity and MMP expression. This result was re-confirmed in the HSC3 oral squamous carcinoma cell line.CONCLUSION:
Targeting hTERT may lead to novel therapeutic approaches.Palavras-chave
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Neoplasias Bucais
/
Carcinoma de Células Escamosas
/
Queratinócitos
/
Movimento Celular
/
Transformação Celular Viral
/
Telomerase
Tipo de estudo:
Prognostic_studies
Limite:
Animals
/
Humans
/
Male
Idioma:
En
Revista:
Anticancer Res
Ano de publicação:
2014
Tipo de documento:
Article