Dietary protein intake affects amino acid and acylcarnitine metabolism in infants aged 6 months.
J Clin Endocrinol Metab
; 100(1): 149-58, 2015 Jan.
Article
em En
| MEDLINE
| ID: mdl-25368978
ABSTRACT
CONTEXT The protective effect of breast-feeding against later obesity may be explained by the lower protein content compared with formula milk. However, the metabolic mechanisms remain unknown. OBJECTIVE:
We studied the metabolic response to a higher or lower protein supply in infancy. DESIGN ANDSETTING:
The Childhood Obesity Project study is a double-blind, randomized, multicenter intervention trial. Infants were randomized to receive a higher (HP) or lower protein (LP) content infant formula or were breast-fed. PATIENTS ANDINTERVENTIONS:
Plasma samples of 691 infants who received formula milk with different protein content (HP, 2.05 g per 100 mL; LP, 1.25 g per 100 mL) or were breast-fed were collected. MAIN OUTCOMEMEASURES:
Changes in plasma amino acid and acylcarnitine concentrations of 6-month-old infants according to different dietary protein supply were determined by liquid chromatography coupled to tandem mass spectrometry.RESULTS:
Twenty-nine metabolites differed significantly between the formula groups. Branched-chain amino acids (BCAAs) were the most discriminant metabolites. Their degradation products, the short-chain acylcarnitines C3, C4, and C5, were also significantly elevated in the HP group. A breakpoint analysis confirmed that with increasing BCAAs, the ratio between acylcarnitines and BCAAs decreases. Long-chain acylcarnitines were decreased in HP infants.CONCLUSIONS:
BCAAs seem to play a pivotal role in the effect of a high-protein diet on ß-oxidation and fat storage. We provide new evidence for a possible saturation of the BCAA degradation pathway that may represent the mechanism by which high-protein intake affects the metabolic regulation. Moreover, it appears to inhibit the initial step of the ß-oxidation, thus leading to high early weight gain and body fat deposition.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Peso Corporal
/
Proteínas Alimentares
/
Carnitina
/
Aminoácidos
Tipo de estudo:
Clinical_trials
/
Etiology_studies
Limite:
Female
/
Humans
/
Infant
/
Male
Idioma:
En
Revista:
J Clin Endocrinol Metab
Ano de publicação:
2015
Tipo de documento:
Article