A reverse passive haemagglutination test for detection of Japanese encephalitis virus antigens in cerebrospinal fluid.
J Virol Methods
; 23(3): 291-8, 1989 Mar.
Article
em En
| MEDLINE
| ID: mdl-2541154
ABSTRACT
A rapid sensitive and specific reverse passive haemagglutination test (RPHA) was developed for the detection of Japanese encephalitis virus (JEV) antigens in human cerebrospinal fluid (CSF). Sheep red cells were sensitized with five monoclonal antibodies (109, 112, 203, 204 and 301) reactive with envelope glycoprotein of JEV. Viral antigens were detected in CSF from 35 of 58 (60%) clinical cases of JE when the five MAb coated cells were used in combination. An IgM capture ELISA detected JEV specific antibodies in CSF among 52 of these 58 cases (90%). While 29 specimens contained both antigen and IgM antibodies, 23 had only IgM antibodies and 6 had only antigen. RPHA proved valuable for detection of viral antigens in CSF samples obtained within 10 days after onset of clinical symptoms. Amongst the five MAbs used, the individual antigen detection rates were 44, 12, 43, 12 and 36%, for MAbs 109, 112, 203, 204 and 301, respectively.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Testes de Hemaglutinação
/
Proteínas do Envelope Viral
/
Vírus da Encefalite Japonesa (Espécie)
/
Antígenos Virais
Tipo de estudo:
Diagnostic_studies
Limite:
Animals
/
Humans
Idioma:
En
Revista:
J Virol Methods
Ano de publicação:
1989
Tipo de documento:
Article
País de afiliação:
Índia