Ligand-engaged TCR is triggered by Lck not associated with CD8 coreceptor.

Casas, Javier; Brzostek, Joanna; Zarnitsyna, Veronika I; Hong, Jin-sung; Wei, Qianru; Hoerter, John A H; Fu, Guo; Ampudia, Jeanette; Zamoyska, Rose; Zhu, Cheng; Gascoigne, Nicholas R J

Casas, Javier; Brzostek, Joanna; Zarnitsyna, Veronika I; Hong, Jin-sung; Wei, Qianru; Hoerter, John A H; Fu, Guo; Ampudia, Jeanette; Zamoyska, Rose; Zhu, Cheng; Gascoigne, Nicholas R J.

Afiliação

Casas J; 1] Department of Microbiology, Yong Loo Lin School of Medicine, National University Health System, National University of Singapore, 5 Science Drive 2, Singapore 117545, Singapore [2] Department of Immunology and Microbial Sciences, The Scripps Research Institute, 10550 N. Torrey Pines Road, La Joll
Brzostek J; 1] Department of Microbiology, Yong Loo Lin School of Medicine, National University Health System, National University of Singapore, 5 Science Drive 2, Singapore 117545, Singapore [2] Department of Immunology and Microbial Sciences, The Scripps Research Institute, 10550 N. Torrey Pines Road, La Joll
Zarnitsyna VI; Wallace H Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, Georgia 30332, USA.
Hong JS; George W Woodruff School of Mechanical Engineering, Georgia Institute of Technology and Emory University, Atlanta, Georgia 30332, USA.
Wei Q; Department of Microbiology, Yong Loo Lin School of Medicine, National University Health System, National University of Singapore, 5 Science Drive 2, Singapore 117545, Singapore.
Hoerter JA; Department of Immunology and Microbial Sciences, The Scripps Research Institute, 10550 N. Torrey Pines Road, La Jolla, California 92037, USA.
Fu G; Department of Immunology and Microbial Sciences, The Scripps Research Institute, 10550 N. Torrey Pines Road, La Jolla, California 92037, USA.
Ampudia J; Department of Immunology and Microbial Sciences, The Scripps Research Institute, 10550 N. Torrey Pines Road, La Jolla, California 92037, USA.
Zamoyska R; Institute of Immunology and Infection Research, University of Edinburgh, Edinburgh EH9 3FL, Scotland, UK.
Zhu C; 1] Wallace H Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, Georgia 30332, USA [2] George W Woodruff School of Mechanical Engineering, Georgia Institute of Technology and Emory University, Atlanta, Georgia 30332, USA.
Gascoigne NR; 1] Department of Microbiology, Yong Loo Lin School of Medicine, National University Health System, National University of Singapore, 5 Science Drive 2, Singapore 117545, Singapore [2] Department of Immunology and Microbial Sciences, The Scripps Research Institute, 10550 N. Torrey Pines Road, La Joll

Nat Commun ; 5: 5624, 2014 Nov 27.

Article em En | MEDLINE | ID: mdl-25427562

ABSTRACT

ABSTRACT

The earliest molecular events in T-cell recognition have not yet been fully described, and the initial T-cell receptor (TCR)-triggering mechanism remains a subject of controversy. Here, using total internal reflection/Forster resonance energy transfer microscopy, we observe a two-stage interaction between TCR, CD8 and major histocompatibility complex (MHC)-peptide. There is an early (within seconds) interaction between CD3Î¶ and the coreceptor CD8 that is independent of the binding of CD8 to MHC, but that requires CD8 association with Lck. Later (several minutes) CD3Î¶-CD8 interactions require CD8-MHC binding. Lck can be found free or bound to the coreceptor. This work indicates that the initial TCR-triggering event is induced by free Lck.

Assuntos

Complexo CD3/metabolismo; Antígenos CD8/metabolismo; Proteína Tirosina Quinase p56(lck) Linfócito-Específica/metabolismo; Animais; Complexo CD3/genética; Antígenos CD8/genética; Feminino; Proteínas de Homeodomínio/genética; Proteínas de Homeodomínio/metabolismo; Ligantes; Proteína Tirosina Quinase p56(lck) Linfócito-Específica/genética; Complexo Principal de Histocompatibilidade; Masculino; Camundongos; Camundongos Knockout; Ligação Proteica; Receptores de Antígenos de Linfócitos T/genética; Receptores de Antígenos de Linfócitos T/metabolismo; Sinapses/enzimologia; Sinapses/genética; Sinapses/metabolismo; Linfócitos T/metabolismo

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Antígenos CD8 / Complexo CD3 / Proteína Tirosina Quinase p56(lck) Linfócito-Específica Tipo de estudo: Risk_factors_studies Limite: Animals Idioma: En Revista: Nat Commun Assunto da revista: BIOLOGIA / CIENCIA Ano de publicação: 2014 Tipo de documento: Article

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