miR-216a regulates snx5, a novel notch signaling pathway component, during zebrafish retinal development.
Dev Biol
; 400(1): 72-81, 2015 Apr 01.
Article
em En
| MEDLINE
| ID: mdl-25645681
ABSTRACT
Precise regulation of Notch signaling is essential for normal vertebrate development. Mind bomb (Mib) is a ubiquitin ligase that is required for activation of Notch by Notch׳s ligand, Delta. Sorting Nexin 5 (SNX5) co-localizes with Mib and Delta complexes and has been shown to directly bind to Mib. We show that microRNA-216a (miR-216a) is expressed in the retina during early development and regulates snx5 to precisely regulate Notch signaling. miR-216a and snx5 have complementary expression patterns. Knocking down miR-216a and/or overexpression of snx5 resulted in increased Notch activation. Conversely, knocking down snx5 and/or miR-216a overexpression caused a decrease in Notch activation. We propose a model in which SNX5, precisely controlled by miR-216a, is a vital partner of Mib in promoting endocytosis of Delta and subsequent activation of Notch signaling.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Retina
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Peixe-Zebra
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Transdução de Sinais
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Regulação da Expressão Gênica no Desenvolvimento
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Proteínas de Peixe-Zebra
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MicroRNAs
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Ubiquitina-Proteína Ligases
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Nexinas de Classificação
Limite:
Animals
Idioma:
En
Revista:
Dev Biol
Ano de publicação:
2015
Tipo de documento:
Article
País de afiliação:
Tunísia