An economical minichamber for immunohistochemical incubation.
J Histochem Cytochem
; 33(2): 162-4, 1985 Feb.
Article
em En
| MEDLINE
| ID: mdl-2578500
ABSTRACT
A simple and economical "slide-minichamber" method for incubating tissue sections with antisera in immunohistochemical (peroxidase-antiperoxidase) staining procedures is described. The technique requires only materials routinely used in the laboratory. The method permits prolonged incubation of tissue sections with antiserum at 4 degrees C or at room temperature, use of small quantities of antiserum, and simultaneous incubation of two tissue sections with the same small quantity of antiserum, thereby allowing use of very dilute antisera and conservation of antisera when availability is limited.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Imunoquímica
/
Histocitoquímica
/
Incubadoras
Tipo de estudo:
Health_economic_evaluation
Limite:
Animals
/
Humans
Idioma:
En
Revista:
J Histochem Cytochem
Assunto da revista:
HISTOCITOQUIMICA
Ano de publicação:
1985
Tipo de documento:
Article