Self-priming on the plant viral RNAs during reverse transcription-PCR.
Acta Virol
; 59(1): 92-7, 2015 Mar.
Article
em En
| MEDLINE
| ID: mdl-25790056
ABSTRACT
The occurrence of the primer-independent cDNA synthesis during RT-PCR analysis of human and animal RNA viruses has been well documented. Conversely, there is scant knowledge about this event in plant RNA viruses. Here we show that the primer-independent cDNA synthesis occurs in all eight different plant RNA viruses tested in this study, suggesting a common phenomenon for RT-PCR analysis of plant RNA viruses. Additional experiments indicate that the event is likely contributed to by RNA self-priming, and can be effectively reduced or eliminated through increasing temperature of the RT reaction.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Vírus de Plantas
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Transcrição Reversa
Idioma:
En
Revista:
Acta Virol
Ano de publicação:
2015
Tipo de documento:
Article