Spatial distribution and temporal evolution of DRONPA-fused SNAP25 clusters in adrenal chromaffin cells.
Photochem Photobiol Sci
; 14(5): 1005-12, 2015 May.
Article
em En
| MEDLINE
| ID: mdl-25837695
ABSTRACT
Sub-diffraction imaging of plasma membrane localized proteins, such as the SNARE (Soluble NSF Attachment Protein Receptor) proteins involved in exocytosis, in fixed cells have resulted in images with high spatial resolution, at the expense of dynamical information. Here, we have imaged localized fluorescence bursts of DRONPA-fused SNAP-25 molecules in live chromaffin cells by Total Internal Reflection Fluorescence (TIRF) imaging. We find that this method allows tracking protein cluster dynamics over relatively long times (â¼20 min.), partly due to the diffusion into the TIRF field of fresh molecules, making possible the simultaneous identification of cluster size, location and temporal evolution. The results indicate that the DRONPA-fused SNAP-25 clusters display rich dynamics, going from staying constant to disappearing and reappearing in specific cluster domains within minutes.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Glândulas Suprarrenais
/
Células Cromafins
/
Proteína 25 Associada a Sinaptossoma
/
Proteínas Luminescentes
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
En
Revista:
Photochem Photobiol Sci
Assunto da revista:
BIOLOGIA
/
QUIMICA
Ano de publicação:
2015
Tipo de documento:
Article
País de afiliação:
Dinamarca