A new method and tool for detection and quantification of PM oxidative potential.
Environ Sci Pollut Res Int
; 22(16): 12469-78, 2015 Aug.
Article
em En
| MEDLINE
| ID: mdl-25903194
ABSTRACT
Airborne particulate matter (PM) contains several quinones, which are able to generate reactive oxygen species impacting on cell viability. A method able to detect and quantify PM oxidative potential, based on the cytochrome c (cyt-c) reduction by means of superoxide anion produced through quinones redox cycling in the presence of reducing agents, is here described. Tris(2-carboxyethyl)phosphine resulted to be the most efficient reducing agent among the ones tested. The procedure included rapid particles extraction, followed by two alternative analytical methods, a spectrophotometric assay based on the initial rate of cyt-c reduction at 550 nm, and an amperometric assay, based on self-assembled monolayers modified gold electrodes. The smallest amount of PM needed to obtain an evaluable signal is 2 µg. The described procedure may represent a starting point to develop devices for PM measurements in polluted atmospheric environments.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Fosfinas
/
Quinonas
/
Monitoramento Ambiental
/
Espécies Reativas de Oxigênio
/
Material Particulado
Tipo de estudo:
Diagnostic_studies
Idioma:
En
Revista:
Environ Sci Pollut Res Int
Assunto da revista:
SAUDE AMBIENTAL
/
TOXICOLOGIA
Ano de publicação:
2015
Tipo de documento:
Article